Comparative cytotoxic activity of Convolvulus pluricaulis against human hepatoma cell line (HepG2) and normal cell line (L929) via apoptosis pathways by flow cytometry analysis

Abstract Background The purpose of this study was to use the MTT test assess cytotoxic effects different extracts Convolvulus pluricaulis leaves in vitro . leaf ethanolic extract has been demonstrated trigger apoptosis on HepG2 cancer cell lines, implying anti-cancer activity. Methods cells were grown culture DMEM and incubated with concentrations plant extracts. Survival rates quantified by assays 24 h exposure (640–20 µg/ml) PEE, CHCl 3 E, EAE, ETHE AQE plant, while monitoring changes cellular shapes inverted phase contrast microscopy (PEE—petroleum ether extract, —chloroform EAE—ethyl acetate ETHE—ethanol AQE—aqueous extract). Results showed IC 50 value < 1000 μg/ml > µg/ml L-929 lines. Hence, are non-toxic against normal line L-929. Treatments standard as a control exhibited necrotic features both On basis these findings, because highly effective partial polarity, cytotoxicity apoptotic activities also investigated. line, higher compared AQE, PEE ( P 0 0001) inhibitory concentration values 35.873 that is μg/ml. percentage determined using propidium iodide (PI) staining DNA fragments flow cytometry. Conclusions action indicating it should be studied further for therapies.